Detection of Metallo beta - lactamases among Enterobacteriaceae isolates at a tertiary care hospital, south India

Journal: South East Asia Journal of Medical Sciences

Article type: Research Article

Published: 2020-07-26

Volume: 4; Issue: 3; Pages: 1-5

ISSN: 2522-7165

DOI: 10.5281/zenodo.4019977

Article URL: https://journals.littlebaypublishers.com/article/article-1783008670181-915843775

PDF: https://journals.littlebaypublishers.com/article/article-1783008670181-915843775.pdf

JATS XML: https://journals.littlebaypublishers.com/article/article-1783008670181-915843775.xml

Authors

  1. Smitha Bagali
    Associate Professor, Department of Microbiology, BLDE (DU) Shri B. M. Patil Medical College, Hospital & Research Centre, Vijayapura, Karnatak, India.
    https://orcid.org/0000-0003-1852-7639
  2. Laxmi Kakhandaki
    Associate Professor, Department of Microbiology, BLDE (DU)Shri B. M. Patil Medical College, Hospital & Research Centre, Vijayapura, Karnataka, India.
  3. Rashmi Karigoudar
    Assistant Professor, Department of Microbiology, BLDE (DU) Shri B. M. Patil Medical College, Hospital & Research Centre, Vijayapura, Karnataka, India.
    https://orcid.org/0000-0003-1852-7639
  4. Shivali V Gajul
    Lecturer, Department of Microbiology, BLDE (DU) Shri B. M. PatilMedical College, Hospital & Research Centre, Vijayapura, Karnataka, India.
  5. Prakash G Mantur
    Associate Professor, Department of Medicine, BLDE (DU) ShriB. M. Patil Medical College, Hospital & Research Centre, Vijayapura, Karnataka, India.
  6. Praveen R Shahapur
    Professor, Department of Microbiology, BLDE (DU) Shri B.M. Patil Medical College, Hospital & Research Centre, Vijayapura, Karnataka, India.

Abstract

Background : The spread of carbapenem resistant bacteria has caused grave concern due to the limited choice in antibiotics for treating infections caused by them. The emergence of  metallo beta-lactamse (MBL) producing  gram negative bacilli poses a therapeutic challenge and is of serious concern for infection control in hospital environment. Materials & Methods: A total of 475 non repeat clinical isolates of family Enterobacteriaceae were included in the study. Resistance to imipenem was determined in isolates by disc diffusion & minimum inhibitory concentration (MIC) method. Imipenem resistant  isolates  were tested for MBL production by combined disc diffusion test and modified Hodge test. Results: Out of the 475 Enterobacteriaceae strains,  20 showed resistance to imipenem. MBL activity was detected in all 20 (4.2%) isolates by combined disc diffusion test, in 18 isolates by modified Hodge test. The MBL producing isolates included clinical strains of Klebsiella spp (45%), E. coli (40%), Citrobacter spp (15%).  Majority of the MBL isolates were from Intensive care unit (65%), from patients with comorbid conditions and with invasive devices.  MBL producing isolates showed high level of resistance to aminoglycosides and fluoroquinolones but all were susceptible to colistin. Conclusion : The need of the hour is to detect MBL producing isolates for better patient outcomes, to execute prompt infection control measures and decrease the escalation of resistance. Keywords :  Imipenem resistance, Phenotypic methods, Colistin sensitivity

Keywords

Imipenem resistance; Phenotypic methods; Colistin sensitivity.

Full Text

HTML full text is not available. Refer to the PDF galley.